Aug
10

CLEM observation of the mitochondrial dynamics in mammalian cells combined with focused ion-beam scanning electron microscopy

Date:
Monday, August 10th, 2015, 11:00 AM
Location:
Elmore Auditorium
Speaker:
Keisuke Ohta, PhD, Kurume University, School of Medicine
Satoko Okayama, Graduate Student, Kurume University, School of Medicine

Host:
Electron Microscopy Facility
Phone:
(561) 972-9000
Email:

Event Information

 

“CLEM observation of the mitochondrial dynamics in mammalian cells combined with focused ion-beam scanning electron microscopy” Keisuke Ohta, PhD, Kurume University, School of Medicine

Focused ion-beam scanning electron microscopy (FIB/SEM) has an ability to reconstruct the biological specimen from tissue to subcellular level with a high spatial resolution in 3D. Additionally, the method has a characteristic that suit the correlative observation between light and electron microscopy referred to as CLEM observation. Using this property, we are trying to visualize the 3D organization of mitochondria-ER-associated membranes (MAMs) around a mitochondrion at a moment of fission. The CLEM observation combined light-microscopic live imaging with FIB/SEM reconstruction would be a powerful tool for life science.

 

“Visualization of mitochondrial nucleoid under electron microscopy”

Satoko Okayama, Graduate Student, Kurume University, School of Medicine

As the localization of DNA within the cell is hard to visualize using conventional electron microscopy, ultrastructural localization of the mammalian mtDNA/nucleoid has not been clear yet. In this study, we tried to visualize the localization of the mtDNA/nucleoid at the electron microscopic level using histochemical methods.

 

This seminar is open to faculty, scientists, and students from Max Planck Florida Institute for Neuroscience (MPFI), Florida Atlantic University (FAU), and the Scripps Research Institute Florida, located on FAU’s John D. MacArthur Campus in Jupiter, Florida. If you belong to an institution outside of the Jupiter campus and would like to attend, please send a request to seminars@mpfi.org