“CLEM observation of the mitochondrial dynamics in mammalian cells combined with focused ion-beam scanning electron microscopy” Keisuke Ohta, PhD, Kurume University, School of Medicine
Focused ion-beam scanning electron microscopy (FIB/SEM) has an ability to reconstruct the biological specimen from tissue to subcellular level with a high spatial resolution in 3D. Additionally, the method has a characteristic that suit the correlative observation between light and electron microscopy referred to as CLEM observation. Using this property, we are trying to visualize the 3D organization of mitochondria-ER-associated membranes (MAMs) around a mitochondrion at a moment of fission. The CLEM observation combined light-microscopic live imaging with FIB/SEM reconstruction would be a powerful tool for life science.
“Visualization of mitochondrial nucleoid under electron microscopy”
Satoko Okayama, Graduate Student, Kurume University, School of Medicine
As the localization of DNA within the cell is hard to visualize using conventional electron microscopy, ultrastructural localization of the mammalian mtDNA/nucleoid has not been clear yet. In this study, we tried to visualize the localization of the mtDNA/nucleoid at the electron microscopic level using histochemical methods.