Intercellular Propagation of ERK Activation as Visualized by FRET biosensors

Friday, June 12th, 2015, 3:00 pm
Elmore Auditorium
Michiyuki Matsuda, MD, PhD, Kyoto University
Ryohei Yasuda, PhD
(561) 972-9000

Event Information

Despite the relevance of Ras-ERK signaling cascade as a downstream of growth signal pathways, technical limitations have obscured dynamic activity changes in vivo. By using cell lines stably-expressing FRET biosensors for ERK, we found that cells exhibit stochastic activity pulses with 10 to 20 minutes duration and that such stochastic ERK activity pulses are laterally propagated in a manner dependent on EGF receptors. In living mice expressing the FRET biosensors, we found firework-like propagation of ERK activations in epidermis, which we call Spatial Propagation of Radial ERK Activity Distribution (SPREAD). Our discovery of SPREAD provides a new insight into how ERK activity is regulated in a multicellular context.

This seminar is open to faculty, scientists, and students from Max Planck Florida Institute for Neuroscience (MPFI), Florida Atlantic University (FAU), and the Scripps Research Institute Florida, located on FAU’s John D. MacArthur Campus in Jupiter, Florida. If you belong to an institution outside of the Jupiter campus and would like to attend, please send a request to seminars@mpfi.org