Jun
11

Molecular tattooing: spatial confinement of drug effects

Date:
Thursday, June 11th, 2015, 11:30 am
Location:
Kahn Conference Room
Speaker:
Miklós Képiró, PhD, Eötvös Loránd University -Postdoc Candidate

Host:
Hyungbae Kwon, PhD
Phone:
(561) 972-9000
Email:

Event Information

Until now, the greatest limitation in the application of bioactive compounds has been the inability of confining them specifically to single cells or subcellular regions within the organism. Therefore, we developed a new technology, molecular tattooing, which applies photoinducible drugs below their effective concentrations and covalently attaches them to their targets by cyclic two-photon irradiation, thereby confining drug effects within the irradiated area. Using molecular tattooing, we mapped the role of myosin in the developing lateral line primordium (pLLp) of zebrafish embryos by inhibiting myosin’s activity in specific regions of the pLLp using azidoblebbistatin, a two-photon inducible myosin inhibitor.

This seminar is open to faculty, scientists, and students from Max Planck Florida Institute for Neuroscience (MPFI), Florida Atlantic University (FAU), and the Scripps Research Institute Florida, located on FAU’s John D. MacArthur Campus in Jupiter, Florida. If you belong to an institution outside of the Jupiter campus and would like to attend, please send a request to seminars@mpfi.org