Dr. Mikihiro Shibata joined the Yasuda lab (Department of Neurobiology at Duke University Medical Center, Durham NC) as a post-doctoral fellow in 2011 and moved with the lab to Max Planck Florida Institute as a research fellow in 2013. In the Yasuda lab, he has developed the high-speed atomic force microscopy to observe the morphological changes of living cells. His current focus is to observe the morphological alteration of neurons during synaptic plasticity by real-time and real-space, which has never been observed by the traditional microscopy.
Dr. Shibata received his Ph.D. in the Department of Materials Science and Engineering at Nagoya Institute of Technology in Nagoya, where he studied the role of internal water molecules in ion-pumping proteins. After that, he moved to Kanazawa University as a post-doctoral fellow, where he learned the high-speed atomic force microscopy to observe the conformational changes of proteins. He directly visualized the conformational change of the light-driven proton pumping protein in real-time with nanometer resolution by response of the light by using high-speed atomic force microscopy.
- Nanoscale imaging of living neurons
- Structural plasticity in hippocampal neurons
- Spine formation
- PhD, Nagoya Institute of Technology, Department of Materials Science and Engineering (2007)
- MS, Nagoya Institute of Technology, Department of Materials Science and Engineering (2005)
- BS, Nagoya Institute of Technology, Department of Materials Science and Engineering (2003)
- Mikihiro Shibata*, Takayuki Uchihashi, Toshio Ando and Ryohei Yasuda* (2015) Long-tip high-speed atomic force microscopy for nanometer-scale imaging in live cells. Sci. Rep. 5, 8724 (* Co-corresponding author)
- Hiroki Watanabe, Takayuki Uchihashi, Toshihide Kobashi, Mikihiro Shibata, Jun Nishiyama, Ryohei Yasuda and Toshio Ando (2013) Wide-area scanner for high-speed atomic force microscopy. Rev. Sci. Instrum. 84, 053702.
- Hayato Yamashita, Kiichi Inoue, Mikihiro Shibata, Takayuki Uchihashi, Jun Sasaki, Hideki Kandori and Toshio Ando (2013) Role of trimer-trimer interaction of bacteriorhodopsin studied by optical spectroscopy and high-speed atomic force microscopy. J. Struct. Biol. 184, 2-11.
- Kosuke Muroda, Keisuke Nakashima, Mikihiro Shibata, Makoto Demura and Hideki Kandori (2012) Protein-bound water as the determinant of asymmetric functional conversion between light-driven proton and chloride pumps. Biochemistry 51, 4677-4684.
- Mikihiro Shibata, Takayuki Uchihashi, Hayato Yamashita, Hideki Kandori and Toshio Ando (2011) Structural changes in bacteriorhodopsin in response to alternate illumination observed by high-speed atomic force microscopy. Angew. Chem. Int. Ed. 50: 4410 -13.
- Mikihiro Shibata, Hayato Yamashita, Takayuki Uchihashi, Hideki Kandori and Toshio Ando (2010) High-speed atomic force microscopy shows dynamic molecular processes in photoactivated bacteriorhodopsin. Nature Nanotech. 5, 208-12.